Publication date 2010 - Basic Science
Hendriks JAA, Miclea RL, Schotel R, De Bruijn E, Moroni L, Karperien M, Riesle J, Van Blitterswijk CA

Primary chondrocytes enhance cartilage tissue formation upon co-culture with a range of cell types


Co-culture models have been increasingly used in tissue engineering applications to understand cell–cell interactions and consequently improve regenerative medicine strategies. Aiming at further elucidating cartilage tissue formation, we co-cultured bovine primary chondrocytes (BPCs) with human expanded chondrocytes (HECs), human dermal fibroblasts (HDFs), mouse embryonic stem cells (MESCs), or mouse-3T3 feeder cells (M3T3s) in micromasses. BPCs were either co-cultured (1 : 5 ratio) with all cell types allowing direct cell–cell contacts or as separate micromasses in the same well with HECs. In co-culture groups with direct cell–cell contacts cartilaginous tissue was formed in all experimental groups. In situ hybridization showed that only 16–27% of the cells expressed type II collagen mRNA. Corresponding with the fact that micromasses consisted for approximately 20% only of BPCs, the amount of GAG was similar between 100% BPC micromass and the co-culture groups with HECs and HDFs. Therefore, co-culture micromasses support cartilage tissue formation predominantly originating from primary chondrocytes in direct contact with a variety of cell types. These findings potentially could be applied to optimize cell-therapy treatments for cartilage regeneration.

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2016 - Clinical Study

Allogeneic Mesenchymal Stem Cells Stimulate Cartilage Regeneration and Are Safe for Single-Stage Cartilage Repair in Humans upon Mixture with Recycled Autologous Chondrons

De Weger RA, De Windt TS, Dhert WJA, Nizak R, Saris DBF, Slaper-Cortenbach ICM, Van den Broek MPH, Van Rijen MHP, Vonk LA
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2017 - Clinical Study

Allogeneic MSCs and Recycled Autologous Chondrons Mixed in a One-Stage Cartilage Cell Transplantion: A First-in-Man Trial in 35 Patients

De Windt TS, Nizak R, Saris DBF, Slaper-Cortenbach ICM, Van Rijen MHP, Vonk LA
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